Antioxidant, antihypertensive, antihyperglycemic, and. Antibacterial and antifungal activities from leaf extracts of. Antibacterial activity of ginger zingiber officinale roscoe and. For instance, extract 8 did not demonstrate hi activity in the most recent batch that was used to study the effects of trypsin treatment upon the antiviral activity of extracts, but the preliminary chemical fraction of extract 8 demonstrated hi activity in one of the fractions. Pdf antibacterial activity of the plant extract of. The concentration of extracts in lard varied from 0. The aim of the study was assess the antibacterial effect of some medicinal plant extracts and their synergistic antibiotic and nonantibiotic drugs against escherichia coli, staphylococcus aureus and pseudomonas aeruginosa. Department of pharmacy, khammam college of pharmacy, khammam, andhra pradesh, india. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. Hot hexane extract showed an inhibition zone of 12 mm against pseudomonas aeruginosa.
Study of total phenolic and flavonoid content, antioxidant. Antibacterial activity of some medicinal plant extracts. Scribd is the worlds largest social reading and publishing site. Evaluation of estrogenic activity of plant extracts for the. Antioxidant activity in plant oil plant oils contain unsaturated fatty acids, which are most sensitive to oxidative degradation. Antimicrobial activity of some plant extracts against.
Antisnake venom activity of different extracts of pouzolzia indica against russel viper venom anas ahmed1, k. Oregano extract was found to be the most effective in stabilizing lard, followed by thyme, dittany, marjoram and lavender extracts, in a decreasing order. Screening of in vitro antioxidant activity of methanolic leaf. Today, this plant has emerged as one of the 20 most popular herbal supplements used worldwide. Biochemistry graduates to have results from their final year research projects on the antibacterial activity of plant extracts submitted for publication dilruba meah and aiysha thompson have just finished their biochemistry. These results show that the aqueous extracts of five native plants of the. Biochemistry graduates to have results from their final year. Antioxidant activity and total phenolic compound content of. Extraction and determination of antioxidant activity of withania somnifera dunal abdul qaiyum ansari 1, syed abrar ahmed1, m. Antioxidant properties of the methanol extracts of the leaves. Leaves and stem were used for antibacterial and phytochemical tests for antibacterial activity the each plant part was soaked in 100 ml of aqueous and methanol extracts leaves and stem in three different concentrations 0.
Antioxidant activity of herbaceous plant extracts protect. Extraction and determination of antioxidant activity of. Antimicrobial activity of crude extracts of some plant leaves. Antioxidant activity of some plant extracts of the family. The plant extract residues 50 mg were re dissolved in 2.
The alcoholic extract of the plant exhibits significant inhibitory. Minimum inhibitory concentration mic assay were determined for these two extracts. The antibacterial activity of extracts of nine plant species with good activity against escherichia coli against five other bacteria and cytotoxicity of extracts ishaku leo elisha 1. Sajid and others published phytochemical screening and. It is therefore recommended that the nature and the number of the active antibacterial principles involved in each plant extract be studied in detail. Rajendaran1, durga jaiswal2, hari pratap singh3, ashutosh mishra3, dinesh chandra4, indranil kumar yadav5and d. Leaf extracts were tested on microbes escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, enterococcus faecalis, candida albicans and bacillus subtilis. Issn phytochemical and antimicrobial evaluation of plant. Pdf antibacterial activity of medicinal plant extracts. Karthika abstract the aim of present study was to estimate the total phenolic and flavonoid contents in vitro antioxidant potential of methanolic leaf and root extracts of the herb. Plant diversity is a basic source of food and medicine for.
The dpph radical scavenging activity of each plant extract was calculated as the percentage inhibition. Both the cold and hot extracts of all the seven solvents exhibited inhibition zones against candida albicans. The mic 50 values of these products against the gramnegative organisms varied from 1. This study was based on screening antibacterial activity of the ethanol extract of baeckea frutescens l. The antioxidant activities of methanol extracts of oregano, dittany, thyme, marjoram, spearmint, lavender and basil were tested in lard stored at 75c. Antibacterial and antifungal activities of thespesia populnea. The antibacterial effect of some medicinal plant extracts. Antibacterial activity of aqueous extract of calotropis. Antibacterial activity of ginger zingiber officinale.
Screening of in vitro antioxidant activity of methanolic leaf and root extracts of hypochaeris radicata l. Antibacterial activity of medicinal plant extracts article pdf available in brazilian journal of microbiology 384. Determination of total phenolic compound content total phenol content of aqueous extract. Bioactive aqueous extracts of twelve yucatecan plants. Antibacterial activity of watersoluble extract from pine needles of cedrus deodara. In addition, the possible synergistic effects when. Antibacterial activity of combined medicinal plants. Plant extracts have shown inhibitory effect on the growth of the bacteria studied, although of distinct forms. Antibacterial activity of hot aqueous and methanolic extracts prepared from six plants terminallia chebula, terminallia bellerica, phyllanthus emblica, punica granatum, lawsonia alba and mikania micrantha used in traditional folk medicines of india were screened against five pathogenic bacteria staphylococcus aureus mtcc 2940, bacillus subtilis mtcc 441, escherichia coli mtcc 739, proteus.
Phytochemical composition, antioxidant, and antimicrobial. Assessment of antioxidant activity of plant extracts by. New drugs from plant secondary metabolites are being developed and studied for their antibacterial and anticancer activities 5. Extract showed maximum antibacterial activity against e. Antibacterial, antioxidant activity of ethanolic plant extracts of some. Identification of traditional medicinal plant extracts with. Ethanol extract six grams of the powdered plant material was extracted in a soxhlet extractor using 25ml of 40% ethanol. Commercially available standard antibiotic discs were also used to find out. Phytochemical investigation and biological activity of leaves extract of plant boswelliaserrata t.
Antibacterial activity of the plant extract of symphytum officinale l. The extract was orally administered at 50 and 200 mgkg body weight. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. The aim of the present study is in vitro screening of antioxidant, antibacterial and a cytotoxic activity of the extracts of nerium oleander leaves. Jain5 1department of pharmacognosy, mcops, manipal576104, india. Pdf antimicrobial activity of medicinal plant leaf. Radical scavenging activity was calculated using the following formula. Plant material was collected from hazargangi chiltan national park, quetta and was dried and grounded for further use. Ourmethods used acidic methanol and water extractions from six herbaceousplants, including bidens alba ba, lycium chinense lc. Extraction, isolation and characterization of bioactive. Antimicrobial activity of fagonia cretica plant extracts. The dried plant materials were ground separately to powder. Extracts from a broad spectrum of plant species contain substances that possess antitumor activity 1.
Antimicrobial activity findings also reveal that the methanol extract led. Evaluation of antibacterial activity of some medicinal plants extracts. To assess the antibacterial activities of coccinia grandis leaf extract on selective. Antibacterial activity, candida albicans, pathogenic microorganism, pseudomonas aeruginosa, thespesia populnea. Antibacterial activity of plant extracts and phytochemicals. Same results were obtained in the present study by using enicostemma hyssopifolium. Two hundred grams of each ground plant materials were shaken separately in methanol for 48 hrs on an orbital shaker. Morphine was administered subcutaneously at a dose of 5mgkg body weight 10. Increasingly, natural products have potential for the treatment of cancer. Successful determination of biologically active compounds from plant material is largely dependent on the type of solvent used in the extraction.
The antimicrobial activity of plant extracts and phytochemicals was evaluated with antibiotic susceptible and resistant microorganisms. Antiinflammatory and analgesic activity of methanolic. Effect of aqueous and alcoholic plant extraction on inhibition of some. The analysis of bioactive compounds present in the plant extracts involving the applications of common phytochemical screening assays, chromatographic techniques such as hplc and, tlc as well as nonchromatographic techniques such as immunoassay and fourier transform infra red ftir are discussed. Chemical composition and antibacterial activity of essential. The objective of this study was to determine the chemical composition and antibacterial activity of essential oils eos of tagetes minuta against three phytopathogenic bacteria pseudomonas savastanoi pv.
It was concluded that ethanol extracts from 70 % of the plants were toxic to cell and only one of the species of combretum duarteanum showed antimicrobial activity. Biosynthesis of nanoparticles is under exploration is due to wide biomedical applications and research interest in nanotechnology. Antibacterial and antifungal activities of plant part extracts against four pathogenic bacteria two grampositive and negative and three pathogenic fungi were investigated by the agar disk diffusion method. Antibacterial potency of combined plant extracts has been tested against bacillus subtilis ifo 3026, sarcina lutea ifo 3232, xanthomonas campestris iam 1671, escherichia coli ifo 3007, klebsiella pneumoniae attc 10031, proteus vulgaris mtcc 321 and pseudomonas denitrificans kacc 32026 by disc diffusion assay. In this work leaf extracts and derived lowpressure column chromatography lpcc fractions of s. The aim of the study is to determine the antibacterial activity of cedrus deodara oil and ricinus communis oil against the gram positive bacteria staphylococcus. They concluded that the whole plant parts of enicostemma axillare. Thephyllanthus amarus was shade dried and extracted with methanol, acetone, chloroform, petroleum ether and hexane. The disk diffusion method is used to evaluate antimicrobial activity of the each plant extract. Oct, 2011 the aims of this study were to further explore the antiinflammatory activity of these plant extracts, commonly used in the skin care industry, and two formulations containing them, to assess their potential antioxidant and antiinflammatory activity using primary human skin fibroblasts. Identification and quantification of plant extracts using.
Antimicrobial activity of crude extracts of three medicinal. The antimicrobial activity of the test organisms to the 11 plant extracts was screened by using the agarwell diffusion method perez et al. Evaluation of estrogenic activity of plant extracts for the potential treatment of menopausal symptoms jianghua liu, joanna e. Disk diffusion could be easily used to screen the antibacterial activity of plant extracts, however results are scarcely comparable with microdilution assay, because of problems related to. Nicolson is a tropical medicinal palnt belonging to rutaceae family.
The plant extracts showed antibacterial activity but not antifungal activity against any of the fungi used. Phytochemical investigation and biological activity of leaves. Antibacterial activity of coccinia grandis leaf extract on selective. The extract was filtered using what man filter paper no. Antioxidant and potential antiinflammatory activity of. The investigation of antimicrobial activity as well as cell toxicity of extracts from 30 plant species against five bacteria species and two fungi species was studied 29. An inoculum suspension was swabbed uniformly to solidified 20 ml muellerhinton agar mha for bacteria and sabouraud dextrose agar sda for fungi and the. In vitro antibacterial activity of several plant extracts and. Screening of antibacterial activity of the medicinal plant. Comparative study of antioxidant properties and total. It was found that methanol extract of phyllanthus amarus showed highest inhibitory activity. Bioreduction of silver nitrate agno 3 and chloroauric acid haucl 4 for the synthesis of silver and gold nanoparticles respectively with the plant extract, mentha piperita lamiaceae.
Thepurpose of this study was to evaluate the antioxidant substances,antioxidant activity, and protection of dna from oxidative damage in humanlymphocytes induced by hydrogen peroxide h2o2. Antioxidant activity dpph free radical scavenging activity of methanolic extract the antioxidant activity of the plant extracts and the standard was assessed on the basis of the radical scavenging effect of the stable 1, 1diphenyl2picrylhydrazyl dpphfree radical activity by modified method. The antibacterial activity of extracts of nine plant. Antibacterial activity of plants extracts against pathogenic bacteria. The resulting extracts were subsequently weighted to produce 0. The antibacterial activity ofphyllanthus amarus was determined by agar well diffusion method. Browns college of pharmacy, khammam, andhra pradesh, india 2. Nov 26, 20 herbaceous plants containing antioxidants can protect against dna damage. Evaluation of antibacterial activity of aqueous extracts of onion and. Plant extract mediated synthesis of silver and gold. Biological screening of various medicinal plant extracts for. Susan srujana1, konduriraveendra babu1,bodavula samba siva rao2 1.
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